Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuCl2 | 1 mM, 10% residual activity | Flavobacterium frigidimaris | |
HgCl2 | 1 mM, no residual activity | Flavobacterium frigidimaris | |
iodoacetate | 1 mM, 16.4% residual activity | Flavobacterium frigidimaris | |
N-ethylmaleimide | 1 mM, no residual activity | Flavobacterium frigidimaris | |
ZnCl2 | 1 mM, 27% residual activity | Flavobacterium frigidimaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
NAD+ | 10°C | Flavobacterium frigidimaris | |
0.029 | - |
NAD+ | 20°C | Flavobacterium frigidimaris | |
0.03 | - |
NAD+ | 30°C | Flavobacterium frigidimaris | |
0.038 | - |
NAD+ | 40°C | Flavobacterium frigidimaris | |
0.269 | - |
L-malate | 20°C | Flavobacterium frigidimaris | |
0.288 | - |
L-malate | 30°C | Flavobacterium frigidimaris | |
0.543 | - |
L-malate | 10°C | Flavobacterium frigidimaris | |
0.74 | - |
L-malate | 40°C | Flavobacterium frigidimaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
4 * 32000, SDS-PAGE | Flavobacterium frigidimaris |
123000 | - |
gel filtration | Flavobacterium frigidimaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium frigidimaris | - |
- |
- |
Flavobacterium frigidimaris KUC-1 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-malate + NAD(P)+ = oxaloacetate + NAD(P)H + H+ | ordered bi-bi mechanism | Flavobacterium frigidimaris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
176 | - |
30°C | Flavobacterium frigidimaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Flavobacterium frigidimaris | oxaloacetate + NADH + H+ | - |
? | |
(S)-malate + NAD+ | - |
Flavobacterium frigidimaris KUC-1 | oxaloacetate + NADH + H+ | - |
? | |
(S)-malate + NADP+ | - |
Flavobacterium frigidimaris | oxaloacetate + NADPH + H+ | - |
? | |
(S)-malate + NADP+ | - |
Flavobacterium frigidimaris KUC-1 | oxaloacetate + NADPH + H+ | - |
? | |
additional information | enzyme shows pro-R stereospecificity for hydrogen transfer at the C4 position of the nicotinamide moiety of the coenzyme. No substrates are: D-malate, malonate, L-glutamate, L-aspartate, D,L-2-hydroxybutanoate, D,L-3-hydroxybutanoate, citrate, maleate, succinate, L-tartrate, L-threonine, L-serine, L-hydroxymalonate, D-glutamate, 2-oxocaproate, 2-oxoisovalerate, glyoxylate, 2-oxoglutarate, 2-oxobutanoate | Flavobacterium frigidimaris | ? | - |
? | |
additional information | enzyme shows pro-R stereospecificity for hydrogen transfer at the C4 position of the nicotinamide moiety of the coenzyme. No substrates are: D-malate, malonate, L-glutamate, L-aspartate, D,L-2-hydroxybutanoate, D,L-3-hydroxybutanoate, citrate, maleate, succinate, L-tartrate, L-threonine, L-serine, L-hydroxymalonate, D-glutamate, 2-oxocaproate, 2-oxoisovalerate, glyoxylate, 2-oxoglutarate, 2-oxobutanoate | Flavobacterium frigidimaris KUC-1 | ? | - |
? | |
oxaloacetate + NADH + H+ | - |
Flavobacterium frigidimaris | L-malate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 32000, SDS-PAGE | Flavobacterium frigidimaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
highest initial velocity | Flavobacterium frigidimaris |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 60 | - |
Flavobacterium frigidimaris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
half-life 26 min | Flavobacterium frigidimaris |
40 | - |
half-life 3 min | Flavobacterium frigidimaris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
41 | - |
NAD+ | 10°C | Flavobacterium frigidimaris | |
41.3 | - |
L-malate | 10°C | Flavobacterium frigidimaris | |
48.4 | - |
L-malate | 20°C | Flavobacterium frigidimaris | |
48.6 | - |
NAD+ | 20°C | Flavobacterium frigidimaris | |
83.2 | - |
L-malate | 30°C | Flavobacterium frigidimaris | |
83.4 | - |
NAD+ | 30°C | Flavobacterium frigidimaris | |
138 | - |
L-malate | 40°C | Flavobacterium frigidimaris | |
139 | - |
NAD+ | 40°C | Flavobacterium frigidimaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
reduction of oxaloacetate | Flavobacterium frigidimaris |
10.5 | - |
oxidation of malate | Flavobacterium frigidimaris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | reduction of oxaloacetate | Flavobacterium frigidimaris |
6.5 | 12 | oxidation of malate | Flavobacterium frigidimaris |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10.5 | - |
Flavobacterium frigidimaris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor: NADPH | Flavobacterium frigidimaris | |
NAD+ | relative activity of NAD+ to NADP+ is 43.9% | Flavobacterium frigidimaris | |
NADH | - |
Flavobacterium frigidimaris | |
NADP+ | relative activity of NAD+ to NADP+ is 43.9% | Flavobacterium frigidimaris |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
289 | - |
L-malate | 30°C | Flavobacterium frigidimaris | |
2790 | - |
NAD+ | 30°C | Flavobacterium frigidimaris |